He studied at 'San Fernando' School of Fine Arts in Madrid. He was one of the pioneers in the vanguard of the 50's as he showed at the First Latin American Biennial in 1951. He carried out many individual exhibitions throughout his life. In 1964, he was the artistic director of the Spanish Pavilion at the World Exhibition Fair in New York.
Manuel Mampaso carried out a very important activity as theatrical designer and costume designer. As far as theatre is concerned, he was responsible for the stage design and clothing of many important plays such as La mordaza by Alfonso Sastre, La camisa by Lauro Olmo, Proceso de Jesús by Ugo Betti and Romance de lobos and Águila de Blasón by Valle-Inclán. He made the designs of the opera Bodas de sangre, first performed at Colón Theatre in Buenos Aires (1965) and made other stage designs in the following plays: El rinoceronte, El Tío Vania, La Celestina, El concierto de San Ovidio, Así es si así os parece, Los bajos fondos, Misericordia and Medea.
In 1987, there was a selective exhibition at Museum Gallery in Houston with paintings and drawings as well as sceneries and theatre and cinematographic designs.